TRIB3 Polyclonal Antibody
Catalogue Numbers: BS60451-50, BS60451-100
Sizes: 50µl, 100µl
Alternative Name: Tribbles homolog 3; TRB-3; Neuronal cell death-inducible putative kinase; SINK; p65-interacting inhibitor of NF-kappa-B; C20orf97; NIPK; SKIP3; TRB3; Tribbles 3
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q96RU7
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB
All Applications: WB: 1:500~1:1000
Background: TRB-3 (tribbles 3), also called NIPK (neuronal cell death-inducible protein kinase) disrupts insulin signaling by binding directly to Akt kinases and blocking their activation. TRB3 binds to ATF4, inhibiting its transcriptional activation activity, and regulates activation of MAP kinases. In the liver, TRB-3 is a target for PPAR-alpha. Amounts of TRB3 RNA and protein are higher in livers of diabetic mice compared with those in wildtype mice. TRB3 contributes to insulin resistance in individuals with susceptibility to type II diabetes. Highest expression of TRB-3 is in liver, pancreas, peripheral blood leukocytes and bone marrow.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: TRIB3 polyclonal antibody detects endogenous levels of TRIB3 protein.
Bioworld Molecular Weight: ~ 40 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of TRIB3 polyclonal antibody at 1:500 dilution
Lane 1: Hela cell lysate
Lane 2: sp2/0 cell lysate
Lane 3: PC12 cell lysate
Immunogen: A synthetic peptide corresponding to residues in Human TRIB3.
Conjugate: Unconjugated
Modification: Unmodified