Product Description

TRIM28 (C-terminus) Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB0014-50, MB0014-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q13263

Host: Mouse

Reactivity: Human

Applications: WB, IHC ICC

All Applications: WB: 1:1000
ICC: 1:200
IHC: 1:500

Background: Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. Recruitment of SETDB1 induces heterochromatinization. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1. Also corepressor for ERBB4. Inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation. May serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of RB1. Important regulator of CDKN1A/p21(CIP1). Has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger. Also specifically sumoylates IRF7, thereby inhibiting its transactivation activity.

Purification and Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of TRIM28(C-terminus) and does not cross-react with related proteins

Bioworld Molecular Weight: Predicted band size: 100KDa
Observed band size: 100KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot detection of TRIM28 (C-terminus) in 293T and HepG2 cell lysates using TRIM28 (C-terminus) antibody (1:1000 diluted)., IHC of paraffin-embedded human Spleen using anti- TRIM28 (C-terminus) diluted 1/500-1/1000., Immunocytochemistry of HeLa cells using anti- TRIM28 (C-terminus) antibody diluted 1:200.

Alternative Name: E3 SUMO-protein ligase TRIM28;
KRAB-associated protein 1; KAP-1; KRAB-interacting protein 1; KRIP-1; Nuclear corepressor KAP-1; KAP1; TF1B; RNF96; TIF1B; TRIM28

Immunogen: Synthetic peptide, corresponding to the C-terminus of Human TRIM28.

Conjugate: Unconjugated

Modification: Unmodified