TriMethyl-Histone H3 (Lys27) Monoclonal Antibody-MB11844

TriMethyl-Histone H3 (Lys27) Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB11844-50, MB11844-100

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Swiss-Prot: P68431

Host: Rabbit

Reactivity: Human, Rat

Applications: WB, IHC-F, IHC-P, ICC/IF, IP, , ChIP,

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20 ChIP: 1/20

Background: H3 Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 15 kDa; Observed MW: 15 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Histone H3 in C6, Jurkat lysates using TriMethyl-Histone H3 antibody., Immunocytochemistry analysis of TriMethyl-Histone H3 in Hela using TriMethyl-Histone H3 antibody, and DAPI, Immunohistochemistry analysis of paraffin-embedded Human lung cancer using TriMethyl-Histone H3 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Alternative Name: H3K27me3; H3 histone; HIST1H3A; Histone cluster 1; H3a

Immunogen: A synthetic methyl-peptide corresponding to residues surrounding Lys27 of human Histone H3

Conjugate: Unconjugated

Modification: Methylated