Trk B (phospho-Y706) Polyclonal Antibody-BS4201

Trk B (phospho-Y706) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50µl, 100µl

Catalogue Numbers: BS4201-50, BS4201-100

Swiss-Prot: Q16620

Host: Rabbit

Trk B (phospho-Y706) Polyclonal Antibody-BS4201

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human

Applications: WB, IHC, IF

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
IF: 1:50~1:200

Background: The Trk proto-oncogene encodes a tyrosine protein kinase, Trk A, also designated Trk gp140, that serves as a receptor for certain neurotrophic factors including nerve growth factor (NGF) and neurotrophin-3 (NT-3). Trk B is a tyrosine kinase gene highly related to Trk A. Trk B expression is confined to tissues within the central and peripheral nervous systems. The brain-derived neurotrophic factor (BDNF) and NT-3, but not NGF, can induce rapid phosphorylation on tyrosine of Trk B gp145, one of the receptors encoded by Trk B, although BDNF elicits a response at least two orders of magnitude greater than NT-3. Thus it appears that Trk B gp145 may represent a neurotrophic receptor for BDNF and NT-3. The third member of the Trk family of tyrosine kinases, Trk C, encodes a protein designated Trk C gp145 that is preferentially expressed in brain tissue, is equally related to Trk A and Trk B, and is a functional receptor for NT-3.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-Trk B (Y706) polyclonal antibody detects endogenous levels of Trk B protein only when phosphorylated at Tyr706

Bioworld Molecular Weight: ~ 92, 145 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Trk B (phospho-Y706) polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate (40ug)
Lane 2: HEK293T treated with EGF (100ng/ml,5 minutes) whole cell lysate (40ug)
Lane 3: HEK293T treated with EGF (100ng/ml,30 minutes) whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of p-Trk B (Y706) pAb in paraffin-embedded human kidney carcinoma tissue at 1:50.showing cytoplasmic staining. Negative control (the right) Using PBS instead of primary antibody,Secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Alternative Name: BDNF/NT-3 growth factors receptor; GP145-TrkB; Trk-B; Neurotrophic tyrosine kinase receptor type 2; TrkB tyrosine kinase; Tropomyosin-related kinase B; NTRK2; TRKB

Immunogen: Synthetic phosphopeptide derived from human Trk B around the phosphorylation site of Tyrosine 706.

Modification: Phosphorylation

Conjugate: Unconjugated