TrkA Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS91373-50, BS91373-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P04629(Human) Q3UFB7(Mouse) P35739(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC, IF
All Applications: WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Background: The family of Trk receptor tyrosine kinases consists of TrkA, TrkB, and TrkC. While the sequence of these family members is highly conserved, they are activated by different neurotrophins: TrkA by NGF, TrkB by BDNF or NT4, and TrkC by NT3. Neurotrophin signaling through these receptors regulates a number of physiological processes, such as cell survival, proliferation, neural development, and axon and dendrite growth and patterning. In the adult nervous system, the Trk receptors regulate synaptic strength and plasticity. TrkA regulates proliferation and is important for development and maturation of the nervous system. Point mutations, deletions, and chromosomal rearrangements (chimeras) cause ligand-independent receptor dimerization and activation of TrkA. TrkA is activated in many malignancies including breast, ovarian, prostate, and thyroid carcinomas. Research studies suggest that expression of TrkA in neuroblastomas may be a good prognostic marker as TrkA signals growth arrest and differentiation of cells originating from the neural crest.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: TrkA polyclonal antibody detects endogenous levels of TrkA protein.
Bioworld Molecular Weight: 145 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of TrkA on different lysates using anti-TrkA antibody at 1/1,000 dilution.
Positive control: Lane 1: Mouse brain Lane 2: SH-SY-5Y, ICC staining TrkA in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: Trk-A, NTRK1, gp140trk, High affinity nerve growth factor receptor, High affinity nerve growth factor receptor precursor, MTC, Neurotrophic tyrosine kinase receptor type 1, NTRK1, NTRK1, Oncogene TRK, p14-TrkA, p140 TrkA, p140-TrkA, Slow nerve growth, Trk A, TRK, Trk-A, TRK1, TRK1-transforming tyrosine kinase protein, Tropomyosin-related kinase A, Tyrosine kinase receptor A, Tyrosine kinase receptor,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified