Product Description

TrkA Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91374-50, BS91374-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P04629(Human) Q3UFB7(Mouse) P35739(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

All Applications: WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200

Background: The Trk proto-oncogene encodes a tyrosine protein kinase, Trk A, also designated Trk gp140, that serves as a receptor for certain neurotrophic factors including nerve growth factor (NGF) and neurotrophin-3 (NT-3). Trk B is a tyrosine kinase gene highly related to Trk A. Trk B expression is confined to tissues within the central and peripheral nervous systems. The brain-derived neurotrophic factor (BDNF) and NT-3, but not NGF, can induce rapid phosphorylation on Tyrosine of Trk B gp145, one of the receptors encoded by Trk B, although BDNF elicits a response at least two orders of magnitude greater than NT-3. Thus it appears that Trk B gp145 may represent a neurotrophic receptor for BDNF and NT-3. The third member of the Trk family of tyrosine kinases, Trk C, encodes a protein designated Trk C gp145 that is preferentially expressed in brain tissue, is equally related to Trk A and Trk B and is a functional receptor for NT-3.

Purification and Purity: Protein affinity purified.

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: TrkA polyclonal antibody detects endogenous levels of TrkA protein.

Bioworld Molecular Weight: Predicted band size 87 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of TrkA on SHSY5Y (1) and SHG-44 (2) cell lysates using anti-TrkA antibody at 1/200 dilution., ICC staining TrkA in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: Trk-A, NTRK1, MTC, TRK, TRKA, gp140trk, High affinity nerve growth factor receptor, High affinity nerve growth factor receptor precursor, MTC, Neurotrophic tyrosine kinase receptor type 1, NTRK1, NTRK1, Oncogene TRK, p14-TrkA, p140 TrkA, p140-TrkA, Slow nerve growth, Trk A, TRK, Trk-A, TRK1, TRK1-transforming tyrosine kinase protein, Tropomyosin-related kinase A, Tyrosine kinase receptor A, Tyrosine kinase receptor,

Immunogen: Recombinant protein

Conjugate: Unconjugated

Modification: Unmodified