Product Description

TrkB (Phospho-Y817) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS94070-50, BS94070-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q16620(Human) P15209(Mouse) Q63604(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC, IP,

All Applications: WB:1:1,000-1:5,000
ICC:1:50-1:200
IHC:1:50-1:200

Background: The Trk proto-oncogene encodes a tyrosine protein kinase, Trk A, also designated Trk gp140, that serves as a receptor for certain neurotrophic factors including nerve growth factor (NGF) and neurotrophin-3 (NT-3). Trk B is a tyrosine kinase gene highly related to Trk A. Trk B expression is confined to tissues within the central and peripheral nervous systems. The brain-derived neurotrophic factor (BDNF) and NT-3, but not NGF, can induce rapid phosphorylation on tyrosine of Trk B gp145, one of the receptors encoded by NTRK2, although BDNF elicits a response at least two orders of magnitude greater than NT-3. Thus it appears that Trk B gp145 may represent a neurotrophic receptor for BDNF and NT-3. The third member of the Trk family of tyrosine kinases, Trk C, encodes a protein designated Trk C gp145 that is preferentially expressed in brain tissue, is equally related to Trk A and Trk B and is a functional receptor for NT-3.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: TrkB (Phospho-Y817) polyclonal antibody detects endogenous levels of TrkB protein only when phosphorylated at Y817.

Bioworld Molecular Weight: 145 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of phospho -TrkB(Y817) on mouse brain lysates using anti- phospho -TrkB(Y817) antibody at 1/1,000 dilution., ICC staining phospho -TrkB(Y817) in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: Trk-B, NTRK2,

Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Tyr817 of human TrkB.

Conjugate: Unconjugated

Modification: Phosphorylation