TRMT61B Polyclonal Antibody-BS71068

TRMT61B Polyclonal Antibody

Sizes: 50 µl, 100 µl

Catalogue Numbers: BS71068-50, BS71068-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9BVS5

Host: Rabbit

Reactivity: Human

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: Methyltransferase that catalyzes the formation of N(1-methyladenine at position 58 (m1A58 in various tRNAs in mitochondrion, including tRNA(Leu (deciphering codons UUA or UUG, tRNA(Lys and tRNA(Ser (deciphering codons UCA, UCU, UCG or UCC. Catalyzes the formation of 1-methyladenosine at position 947 of mitochondrial 16S ribosomal RNA and this modification is most likely important for mitoribosomal structure and function. In addition to tRNA N(1-methyltransferase activity, also acts as a mRNA N(1-methyltransferase by mediating methylation of adenosine residues at the N(1 position of MT-ND5 mRNA, leading to interfere with mitochondrial translation.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of variousLysates, using TRMT61B antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.

Bioworld Molecular Weight: 52kDa

Specificity: Polyclonal Antibodies

Alternative Name: TRMT61B

Immunogen: Recombinant fusion protein of human TRMT61B(NP_060380.3).

Conjugate: Unconjugated

Modification: Unmodified