TRNT1 Polyclonal Antibody Sizes: 50µl, 100µl Catalogue Numbers: BS77417-50, BS77417-100 Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Swiss-Prot: Q96Q11 Host: Rabbit Reactivity: Human, Mouse, Rat Applications: WB All Applications: WB,1:500 - 1:2000 Background: The protein encoded by this gene is a CCA-adding enzyme which belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. This essential enzyme functions by catalyzing the addition of the conserved nucleotide triplet CCA to the 3' terminus of tRNA molecules. Mutations in this gene result in sideroblastic anemia with B-cell immunodeficiency, periodic fevers, and developmental delay. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014] Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity: Unmodification Extra Notes: Western blot analysis of extracts of various cell lines, using TRNT1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s. Note: For research use only, not for use in diagnostic procedure. Bioworld Molecular Weight: 50kDa Alternative Name: CCA1; CGI-47; MtCCA; RPEM; SIFD; TRNT1 Immunogen: Recombinant fusion protein of human TRNT1(NP_886552.2). Conjugate: Unconjugated Modification: Unmodified