Troponin I3 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS91378-50, BS91378-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P19429(Human) P48787(Mouse)
Host: Rabbit
Reactivity: Human, Mouse
Applications: WB, ICC/IF, IHC, IP,
All Applications: WB:1:1,000-1:5,000
ICC:1:50-1:200
IHC:1:50-1:200
Background: Actin is a highly conserved protein that is expressed in all eukaryotic cells. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. Myosin is a hexamer composed of two heavy chains (MHC) and four light chains (MLC); it interacts with Actin to generate the force for diverse cellular movements, including cytokinesis, phagocytosis and muscle contraction. Troponin facilitates the interaction between Actin and Myosin by binding to calcium. Troponin comprises at least two subunits, which are divergent in cardiac muscle, fast skeletal muscle and slow skeletal muscle. Structures of skeletal muscle troponin are composed of Troponin C (the sensor), Troponin I (the regulator) and Troponin T (the link to the muscle thin filament). Troponin C is dumbbell-shaped and has a hydrophobic pocket that increases the contractile force of muscle fibers. Troponin C has two isoforms: fast and slow. Fast Troponin C has two calcium binding sites while slow/cardiac Troponin C has a single calcium binding site.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: Troponin I3 polyclonal antibody detects endogenous levels of Troponin I3 protein.
Bioworld Molecular Weight: 24 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Troponin I on human heart lysates using anti-Troponin I antibody at 1/1,000 dilution., ICC staining Troponin I in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: TNNI3, cardiac muscle, Cardiac troponin I, cardiomyopathy, dilated 2A (autosomal recessive), Cardiomyopathy, familial hypertrophic, 7, included, CMD1FF, CMD2A, CMH7, cTnI, Familial hypertrophic cardiomyopathy 7, MGC116817, RCM1, Tn1, Tni, TNN I3, TNNC 1, TNNC1, TNNI3, TNNI3, Troponin I, Troponin I cardiac, Troponin I cardiac muscle, Troponin I cardiac muscle isoform, Troponin I type 3 cardiac, troponin I, cardiac 3, TroponinI, Ttroponin I type 3 (cardiac),
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified