Troponin T1 Polyclonal Antibody-BS60260

Troponin T1 Polyclonal Antibody

Catalogue Numbers: BS60260-50, BS60260-100

Sizes: 50µl, 100µl

Alternative Name: Troponin T, slow skeletal muscle; TnTs; Slow skeletal muscle troponin T; sTnT; TNNT1; TNT; Troponin T-SS

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P13805

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Actin is a highly conserved protein that is expressed in all eukaryotic cells and interacts with Myosin to generate the force for diverse cellular movements, including cytokinesis, phagocytosis and muscle contraction. Troponin facilitates the interaction between Actin and Myosin by binding to calcium. Troponin is made up of at least two subunits, which are divergent in cardiac muscle, fast skeletal muscle and slow skeletal muscle. Structures of skeletal muscle Troponin are composed of Troponin C (the sensor), Troponin I (the regulator) and three Troponin T (the link to the muscle thin filament) proteins, one of which functions as a Tropomyosin-binding protein and is known as Troponin T-SS (Troponin T-Slow Skeletal). Defects in the gene encoding Troponin T-SS are the cause of nemaline myopathy type 5 (NEM5), a form of nemaline myopathy characterized by mild contractures of the shoulders and hips, tremors and respiratory problems that often lead to death. Troponin T-SS is expressed as three isoforms due to alternative splicing events.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Troponin T1 polyclonal antibody detects endogenous levels of Troponin T1 protein.

Bioworld Molecular Weight: ~ 23 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Troponin T1 polyclonal antibody at 1:500 dilution
Lane 1: A549 whole cell lysate
Lane 2: sp2/0 whole cell lysate
Lane 3: Rat skeletal muscel tissue lysate

Immunogen: A synthetic peptide corresponding to residues in Human Troponin T1.

Conjugate: Unconjugated

Modification: Unmodified