Product Description

TSG101 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS6042-50, BS6042-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q99816

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

All Applications: WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200

Background: The transformation of a normal cell to one that is malignant can result from mutations in genes that encode proteins with key regulatory functions. Examples include the retinoblastoma gene product (Rb p110), p53, VHL and APC. Using a novel cloning strategy that allows the isolation of previously uncharacterized genes encoding selectable recessive phenotypes, an additional tumor suppressor gene has been identified. This gene, termed tsg 101 for tumor susceptibility gene 101, encodes a stathmin binding domain protein. When expression of this growth inhibitory gene is blocked in NIH/3T3 cells using antisense mRNA, the cells exhibit a transformed phenotype and are tumorigenic in SL6 mice.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: TSG101 polyclonal antibody detects endogenous levels of TSG101 protein.

Bioworld Molecular Weight: ~ 48 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of TSG101 polyclonal antibody at 1:500 dilution
Lane 1: Hela whole cell lysate (40ug)
Lane 2: The Brain cord tissue lysate of Mouse (40ug)
Lane 3: The Brain tissue lysate of Rat (40ug), Immunofluorescence analysis of Hela cells using TSG101 antibody at dilution of 1:50., Immunohistochemistry of paraffin-embedded Human Brain using TSG101 antibody at dilution of 1:50.

Alternative Name: Tumor susceptibility gene 101 protein; ESCRT-I complex subunit TSG101; TSG101

Immunogen: Synthetic peptide, corresponding to Human TSG101.

Conjugate: Unconjugated

Modification: Unmodified