Product Description

TSG101 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91381-50, BS91381-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q99816(Human) Q61187(Mouse) Q6IRE4(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC, FC

All Applications: WB:1:1,000-1:2,000
ICC:1:100-1:500
IHC:1:50-1:200
FC:1:50-1:100

Background: The transformation of a normal cell to one that is malignant can result from mutations in genes that encode proteins with key regulatory functions. Examples include the retinoblastoma gene product (Rb p110), p53, VHL and APC. Using a novel cloning strategy that allows the isolation of previously uncharacterized genes encoding selectable recessive phenotypes, an additional tumor suppressor gene has been identified. This gene, termed tsg 101 for tumor susceptibility gene 101, encodes a stathmin binding domain protein. When expression of this growth inhibitory gene is blocked in NIH/3T3 cells using antisense mRNA, the cells exhibit a transformed phenotype and are tumorigenic in SL6 mice.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: TSG101 polyclonal antibody detects endogenous levels of TSG101 protein.

Bioworld Molecular Weight: 44 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of TSG101 on different lysates using anti-TSG101 antibody at 1/1,000 dilution.
Positive control: Lane 1: NIH/3T3 Lane 2: Hela Lane 3: K562 Lane 4: Jurkat Lane 5: A431, ICC staining TSG101 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: TSG101, ESCRT I complex subunit TSG101, ESCRT-I complex subunit TSG101, TS101, TSG 10, TSG 101, TSG10, Tsg101, Tumor susceptibility gene 10, Tumor susceptibility gene 101, Tumor susceptibility gene 101 protein, Tumor susceptibility protein, Tumor susceptibility protein isoform 3, VPS 23, VPS23,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified