TTC11/FIS1 Polyclonal Antibody-BS7881

TTC11/FIS1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS7881-50, BS7881-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9Y3D6

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC, IP,

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200 | IP,1:50 - 1:200

Background: The balance between fission and fusion regulates the morphology of mitochondria. TTC11 is a component of a mitochondrial complex that promotes mitochondrial fission (James et al., 2003 [PubMed 12783892]).[supplied by OMIM, Mar 2008]

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 17KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of 293T cells, using TTC11/FIS1 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s., Confocal immunofluorescence analysis of HeLa cells using TTC11/FIS1 antibody at dilution of 1:50. Blue: DAPI for nuclear staining., Immunoprecipitation analysis of 200ug extracts of MCF7 cells using 3ug TTC11/FIS1 antibody. Western blot was performed from the immunoprecipitate using TTC11/FIS1 antibody at a dilution of 1:1000.

Alternative Name: CGI-135; TTC11; FIS1; TTC11 / FIS1; fission

Immunogen: Recombinant fusion protein of human TTC11/FIS1(NP_057152.2).

Conjugate: Unconjugated

Modification: Unmodified