Product Description

TTN Polyclonal Antibody

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50µl, 100µl

Catalogue Numbers: BS76925-50, BS76925-100

Swiss-Prot: Q8WZ42

Host: Rabbit

TTN Polyclonal Antibody-BS76925

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Mouse, Rat

Applications: IF/ICC

All Applications: IF/ICC,1:50 - 1:200

Background: This gene encodes a large abundant protein of striated muscle. The product of this gene is divided into two regions, a N-terminal I-band and a C-terminal A-band. The I-band, which is the elastic part of the molecule, contains two regions of tandem immunoglobulin domains on either side of a PEVK region that is rich in proline, glutamate, valine and lysine. The A-band, which is thought to act as a protein-ruler, contains a mixture of immunoglobulin and fibronectin repeats, and possesses kinase activity. An N-terminal Z-disc region and a C-terminal M-line region bind to the Z-line and M-line of the sarcomere, respectively, so that a single titin molecule spans half the length of a sarcomere. Titin also contains binding sites for muscle associated proteins so it serves as an adhesion template for the assembly of contractile machinery in muscle cells. It has also been identified as a structural protein for chromosomes. Alternative splicing of this gene results in multiple transcript variants. Considerable variability exists in the I-band, the M-line and the Z-disc regions of titin. Variability in the I-band region contributes to the differences in elasticity of different titin isoforms and, therefore, to the differences in elasticity of different muscle types. Mutations in this gene are associated with familial hypertrophic cardiomyopathy 9, and autoantibodies to titin are produced in patients with the autoimmune disease scleroderma.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Unmodification

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunofluorescence analysis of rat heart using TTN antibody at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of mouse heart using TTN antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: TTN; CMD1G; CMH9; CMPD4; EOMFC; HMERF; LGMD2J; MYLK5; TMD; SALMY; titin

Immunogen: Recombinant fusion protein of human TTN(NP_596870.2).

Modification: Unmodified

Conjugate: Unconjugated