TTR Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS6039-50, BS6039-100
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P02766
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC, IF/ICC
All Applications: WB,1:500 - 1:2000 | IHC,1:50 - 1:100 | IF/ICC,1:50 - 1:100
Background: This gene encodes transthyretin, one of the three prealbumins including alpha-1-antitrypsin, transthyretin and orosomucoid. Transthyretin is a carrier protein; it transports thyroid hormones in the plasma and cerebrospinal fluid, and also transports retinol (vitamin A) in the plasma. The protein consists of a tetramer of identical subunits. More than 80 different mutations in this gene have been reported; most mutations are related to amyloid deposition, affecting predominantly peripheral nerve and/or the heart, and a small portion of the gene mutations is non-amyloidogenic. The diseases caused by mutations include amyloidotic polyneuropathy, euthyroid hyperthyroxinaemia, amyloidotic vitreous opacities, cardiomyopathy, oculoleptomeningeal amyloidosis, meningocerebrovascular amyloidosis, carpal tunnel syndrome, etc.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Polyclonal Antibodies
Bioworld Molecular Weight: 16KDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of extracts of HepG2 cells, using TTR antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 180s., Western blot analysis of extracts of various cell lines, using TTR antibody at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 180s., Immunohistochemistry of paraffin-embedded mouse lung using TTR antibody at dilution of 1:100. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol., Immunofluorescence analysis of human liver cancer using TTR Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Alternative Name: TTR; CTS; CTS1; HEL111; HsT2651; PALB; TBPA
Immunogen: Recombinant fusion protein of human TTR(NP_000362.1).
Conjugate: Unconjugated
Modification: Unmodified