Tubulinγ Polyclonal Antibody-BS91386

Tubulinγ Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91386-50, BS91386-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P23258(Human) Q9NRH3(Human) P83887(Mouse) Q8VCK3(Mouse) P83888(Rat) P23330(Xenopus laevis)

Host: Rabbit

Reactivity: Human, Mouse, Rat, Monkey, Chicken, Hamster, Cow, Dog, Fish, Xenopus tropicalis

Applications: WB, ICC/IF, IHC, IP, , FC

All Applications: WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: Tubulin is a major cytoskeleton component that has five distinct forms, designated α, β, γ, δ and e Tubulin. α and β Tubulins form heterodimers which multimerize to form a microtubule filament. Multiple β Tubulin isoforms (β1, β2, β3, β4, β5, β6 and β8) have been characterized and are expressed in mammalian tissues. β1 and β4 are present throughout the cytosol, β2 is present in the nuclei and nucleoplasm, and β3 is a neuron-specific cytoskeletal protein. γ Tubulin forms the gammasome, which is required for nucleating microtubule filaments at the centrosome. Both δ Tubulin and e Tubulin are associated with the centrosome. δ Tubulin is a homolog of the Chlamydomonas δ Tubulin Uni3 and is found in association with the centrioles, whereas e Tubulin localizes to the pericentriolar material. e Tubulin exhibits a cell-cycle-specific pattern of localization, first associating with only the older of the centrosomes in a newly duplicated pair and later associating with both centrosomes.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Tubulinγ polyclonal antibody detects endogenous levels of Tubulinγ protein.

Bioworld Molecular Weight: 51 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of gamma Tubulin on different lysates using anti-gamma Tubulin antibody at 1/1,000 dilution.
Positive control: Lane 1: A431 Lane 2: Jurkat Lane 3: Mouse brain, ICC staining gamma Tubulin in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: GCP-1, TUBG1, TUBG2, Gamma 1 tubulin, Gamma 2 tubulin, Gamma Tubulin 1, Gamma Tubulin 2, Gamma tubulin complex component 1, Gamma-2-tubulin, GCP 1, GCP1, MGC131994, TBG2, TUBG, TUBG1, TUBG2, TUBGCP1, Tubulin gamma 1 chain, Tubulin gamma 2 chain, Tubulin gamma complex-associated protein 1, Tubulin gamma-2 chain, tubulin, gamma 1, tubulin, gamma 2, tubulin, gamma polypeptide, Xgam,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified