TWEAK (P73) Polyclonal Antibody-BS2454

TWEAK (P73) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS2454-50, BS2454-100

Sizes: 50µl, 100µl

Swiss-Prot: O43508

Host: Rabbit

Reactivity: Human

Applications: WB, IHC, IF

Background: TWEAK is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein is a ligand for the FN14/TWEAKR receptor. It is widely expressed, has high constitutive expression in heart placenta, lung, muscle, kidney, pancreas, and HUVEC. This cytokine has overlapping signaling functions with TNF, but displays a much wider tissue distribution. TWEAK can induce apoptosis via multiple pathways of cell death in a cell type specific manner. This cytokine is also found to promote proliferation and migration of endothelial cells, and thus acts as a regulator of angiogenesis.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: TWEAK (P73) polyclonal antibody detects endogenous levels of TWEAK protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of TWEAK (P73) polyclonal antibody at 1:500 dilution
Lane 1: A549 whole cell lysate (40ug)
Lane 2: U-87MG whole cell lysate (40ug)
Lane 3: HEK293T whole cell lysate (40ug)
Lane 4: A2780 whole cell lysate (40ug)
Lane 5: SP2/0 whole cell lysate (40ug)
Lane 6: C6 whole cell lysate (40ug)
Lane 7: PC12 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of TWEAK (P73) pAb in paraffin-embedded human colon carcinoma tissue at 1:50.showing secreted and cell membrane staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Bioworld Molecular Weight: ~ 27 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Tumor necrosis factor ligand superfamily member 12; APO3 ligand; TNF-related weak inducer of apoptosis; TWEAK; TNFSF12; APO3L; DR3LG; UNQ181/PRO207

Immunogen: Synthetic peptide, corresponding to amino acids 51-100 of Human TWEAK.

Conjugate: Unconjugated

Modification: Unmodified