U1A Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS91394-50, BS91394-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P09012(Human) Q62189(Mouse)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC IF, IHC, IP,
All Applications: WB:1:500-1:1,000
ICC:1:50-1:200
IHC:1:50-1:200
IP:1:50-1:100
Background: SNRPA (small nuclear ribonucleoprotein polypeptide A), also known as U1A (U1 snRNP protein A), is a component of the RNA spliceosome, a complex of proteins that are required for the precise excision of introns from pre-messenger RNA (pre-mRNA). Localizing to the nucleus, SNRPA contains two RRM (RNA recognition motif) domains, namely RRM1 and RRM2, and RRM1 specifically associates with the stem loop II of U1 snRNA (small nuclear RNA). In addition to functioning as a component of the U1 snRNP, SNRPA negatively regulates polyadenylation of SNRPA pre-mRNA, thereby negatively regulating itself. By inhibiting the addition of a polyA tail that would allow the pre-mRNA to mature, SNRPA causes the nuclear exosome degradation of the SNRPA pre-mRNA. At least 16% of cellular SNRPA also functions in an snRNP-free form (SF-A) that complexes with a group of non-snRNP proteins.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Specificity: U1A polyclonal antibody detects endogenous levels of U1A protein.
Bioworld Molecular Weight: 31 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of U1A on different lysates using anti-U1A antibody at 1/500 dilution.
Positive control:
Lane 1: Rat brain
Lane 2: K562
Lane 3: A431
Lane 4: Mouse kidney, ICC staining U1A in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: U1 snRNP A, U1-A, U1A, SNRPA, fc19d01, Mud1, small nuclear ribonucleoprotein polypeptide A, snRNP A, snRNP protein A, SNRPA, SNRPA, U1 small nuclear ribonucleoprotein A, U1 small nuclear RNP specific A, U1 snRNP A, U1 snRNP specific protein A, U1-A, U1A, wu:fc19d01, zgc:77810,
Immunogen: Recombinant protein corresponding to N-terminal human U1A.
Conjugate: Unconjugated
Modification: Unmodified