Product Description

U2AF2/U2AF65 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS7326-50, BS7326-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P26368

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IF/ICC, IP,

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:100 | IP,1:50 - 1:200

Background: U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 54kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using U2AF2/U2AF65 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST., Immunofluorescence analysis of U2OS cells using U2AF2/U2AF65 antibody. Blue: DAPI for nuclear staining., Immunoprecipitation analysis of 200ug extracts of SW620 cells using 1ug U2AF2/U2AF65 antibody. Western blot was performed from the immunoprecipitate using U2AF2/U2AF65 antibody at a dilution of 1:500.

Alternative Name: U2AF2; U2AF65

Immunogen: Recombinant fusion protein of human U2AF2/U2AF65(NP_001012496.1).

Conjugate: Unconjugated

Modification: Unmodified