UBC9 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS91398-50, BS91398-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P63279(Human) P63280(Mouse) P63281(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF IHC, IP, CHIP,
All Applications: WB:1:500-1:1,000
ICC:1:50-1:200
IHC:1:50-1:200
Background: UBC9 is a component of the ubiquitin-mediated proteolytic pathway, which targets proteins for degradation by the 26S proteasome, mediates endocytosis and directs protein subcellular localization. Ub and Ub-like molecules are systematically transferred from E2 conjugating enzymes to the targeted substrate by way of an E3 ubiquitin ligase. UBC9 functions as an E2 ubiquitin conjugating enzyme that preferentially associates with the ubiquitin homolog designated SUMO-1 or sentrin, a component of the sentrinization complex. Characteristic of the E2 family members, UBC9 contains a conserved cysteine residue that is required for the thio ester formation between Ub-like proteins and the E2 member, and it shares a conserved UBC domain. Substrates for UBC9 include transcription factors E12 and E47 and mitotic regulators RanBP2 and RanGAP1, which indicates that UBC9 may regulate various cellular processes including cell cycle progression and differentiation.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: UBC9 polyclonal antibody detects endogenous levels of UBC9 protein.
Bioworld Molecular Weight: 18 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of UBC9 on different lysates using anti-UBC9 antibody at 1/1,000 dilution.
Positive control: Lane 1: HepG2 Lane 2: HUVEC, ICC staining UBC9 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: UBE2I, C358B7.1, p18, SUMO 1 protein ligase, SUMO conjugating enzyme UBC9, SUMO-conjugating enzyme UBC9, SUMO-protein ligase, SUMO1 protein ligase, UBC9, UBC9, UBCE9, Ube2i, Ubiquitin carrier protein 9, Ubiquitin carrier protein, Ubiquitin carrier protein I, Ubiquitin conjugating enzyme 9, Ubiquitin conjugating enzyme E2I (homologous to yeast UBC9), Ubiquitin conjugating enzyme E2I (UBC9 homolog, yeast), Ubiquitin conjugating enzyme UbcE2A, Ubiquitin like protein SUMO 1 conjugating enzyme, Ubiquitin protein ligase E2I, Ubiquitin-conjugating enzyme E2 I, Ubiquitin-protein ligase I,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified