UBE2E2 Polyclonal Antibody-BS76787

UBE2E2 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS76787-50, BS76787-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q96LR5

Host: Rabbit

Reactivity: Mouse, Rat

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: Ube2E2 is an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for ubiquitination of protein substrates. It shares 96% identical amino acid sequence with Ube2E1 and Ube2E3 with the exception of the N-terminal extension that has little in common with the other two E2s. Ube2E2 interacts with ARA54 and RNF8. The RNF8/ Ube2E2 interaction is found to catalyze K48-linked poly-ubiquitination. Ube2E2 may play a role in ubiquitination and subsequent proteasomal degradation of the E3 Ub ligase Parkin.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Extra Notes: Western blot analysis of extracts of various cell lines, using UBE2E2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 180s.

Note: For research use only, not for use in diagnostic procedure.

Bioworld Molecular Weight: 22KDa

Alternative Name: UBE2E2; UBCH8

Immunogen: Recombinant fusion protein of human UBE2E2(NP_689866.1).

Conjugate: Unconjugated

Modification: Unmodified