Product Description

UBE2L3 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS70601-50, BS70601-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P68036

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF/ICC

All Applications: WB,1:1000 - 1:2000 | IHC,1:50 - 1:100 | IF/ICC,1:50 - 1:200

Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Several alternatively spliced transcript variants have been found for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 18kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using UBE2L3 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 60s., Immunohistochemistry of paraffin-embedded rat lung using UBE2L3 Rabbit pAb at dilution of 1:100. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded rat kidney using UBE2L3 Rabbit pAb at dilution of 1:100. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol., Immunofluorescence analysis of HeLa cells using UBE2L3 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: UBE2L3; E2-F1; L-UBC; UBCH7; UbcM4

Immunogen: Recombinant fusion protein of human UBE2L3(NP_001243284.1).

Conjugate: Unconjugated

Modification: Unmodified