Product Description

UBE2Q2 Polyclonal Antibody

Sizes: 50 µl, 100 µl

Catalogue Numbers: BS71401-50, BS71401-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q8WVN8

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:100

Background: Ube2Q2 is an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for ubiquitination of protein substrates. Ube2Q2 is thought to play a key role in regulating mitotic stages in cells. Its discovery was in connection with an arrest in the mitotic cell cycle. Inactivating this E2 prohibits the cell from continuing on past prophase. In cases of head and neck squamou cell carcinaoma, there is an elevated level of Ube2Q2 in the cancerous tissues. Also when the expression levels decreased, the cancerous cells were found to have a resistance to chemotherapeutic agents.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using UBE2Q2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.

Bioworld Molecular Weight: 43kDa

Specificity: Polyclonal Antibodies

Alternative Name: UBE2Q2

Immunogen: Recombinant fusion protein of human UBE2Q2(NP_775740.1).

Conjugate: Unconjugated

Modification: Unmodified