Product Description

UBE3A Polyclonal Antibody

Sizes: 60μL, 120μL, 200μL

Catalogue Numbers: E-AB-62254-60, E-AB-62254-120, E-AB-62254-200

Citations, Manuals and MSDS Available upon request.

Abbreviation: UBE3A

Target Synonym: UBE3A; ANCR; AS; E6-AP; EPVE6AP; HPVE6A

Research Areas: Cell Biology, Epigenetics and Nuclear Signaling, Neuroscience

Conjugation: Unconjugated

Host: Rabbit

Species Reactivity: Human, Mouse, Rat

Application: WB, IHC

Isotype: IgG

Clonality: Polyclonal

UNIProt ID: Q05086

Background: This gene encodes an E3 ubiquitin-protein ligase, part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome, characterized by severe motor and intellectual retardation, ataxia, hypotonia, epilepsy, absence of speech, and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53. Alternative splicing of this gene results in three transcript variants encoding three isoforms with different N-termini. Additional transcript variants have been described, but their full length nature has not been determined.

Concentration: 1mg/mL

Immunogen: Recombinant fusion protein of human UBE3A (NP_570853.1).

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification Method: Affinity purification

Dilution: WB 1:500-1:2000 IHC 1:50-1:200

Calculated MW: 97kDa/100kDa

Observed MW: 110kDa

Storage: -20°C/One year. Avoid freeze / thaw cycles.

Shipping: Ice bag

Research Use Only