UBE3B Polyclonal Antibody - BT-AP09365

UBE3B Polyclonal Antibody

Sizes: 20μL, 50μL, 100μL

Catalogue Numbers: BT-AP09365-20, BT-AP09365-50, BT-AP09365-100

Citations, Manuals and MSDS Available upon request.

Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: e1 ubiquitin-activating enzymes, E2 ubiquitin-conjugating enzymes, and E3 ubiquitin-protein ligases. UBE3B (ubiquitin protein ligase E3B) encodes a member of the E3 ubiquitin-conjugating enzyme family which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme and transfers the ubiquitin to the targeted substrates. A HECT (homology to E6-AP C-terminus) domain in the C-terminus of the longer isoform of this protein is the catalytic site of ubiquitin transfer and forms a complex with E2 conjugases. Shorter isoforms of this protein which lack the C-terminal HECT domain are therefore unlikely to bind E2 enzymes. Alternatively spliced transcript variants encoding distinct isoforms have been identified for UBE3B.

Research Areas: Others

Target Protein: UBE3B

Applications: WB, IHC-p, ELISA

Reactivity: Human

Clonality: Polyclonal

Host: Rabbit

Isotype: IgG

Full Product Name: UBE3B Antibody

Immunogen: The antiserum was produced against synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172. AA range:140-189

Storage: -20°C for 1 year

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration: 1 mg/ml

Molecular Weight (Da): 123070

UniProt Accession: Human: Q7Z3V4; Mouse: Q9ES34

Synonyms: UBE3B; Ubiquitin-protein ligase E3B

GeneID: Human: 89910

Research Use Only