Product Description

UBF-1 (E536) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS1939-50, BS1939-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The transcription of ribosomal RNA genes by RNA polymerase I (Pol I) is tightly coordinated with the growth state of the cell. In addition to Pol I, transcription of ribosomal genes requires the trans-activating factor UBF (upstream binding factor). UBF functions by binding to DNA elements within the RNA gene promoter and enhancer regions and directly associating with Pol I, tethering it to the promoter complex. Two UBF proteins, of 97 kDa and 94 kDa, arise from the same gene as a resμlt of alternative mRNA splicings. UBF activity is regμlated by several dependent casein kinase II phosphorylates at the carboxy terminus of UBF on serine residues. The retinoblastoma susceptibility gene product, Rb, when not bound to E2F family members, inhibits UBF activity. Expression of RNA may also be negatively regμlated by the 70 kDa and 86 kDa Ku antigens.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC

Alternate Names: Nucleolar transcription factor 1, Autoantigen NOR-90, Upstream-binding factor 1, UBF-1, UBTF, UBF, UBF1

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Synthetic peptide, corresponding to amino acids 500-550 of Human UBF-1.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: UBF-1 (E536) polyclonal antibody detects endogenous levels of UBF-1 protein.

Molecular Weight: ~ 90 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P17480

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only