Ubiquitin (1B8) Monoclonal Antibody-MB3275

Ubiquitin (1B8) Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB3275-50, MB3275-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: P0CG47

Host: Mouse

Reactivity: Transfected

Applications: WB, IHC-P

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100

Background: Plays an important role in the ubiquitin-proteasome pathway. Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the activation component E1; the activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, then from E2 to ubiquitin ligase E3 for final delivery to the epsilon-NH2 of the target protein lysine residue.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG1

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Ubiquitin in Hela lysates, 3T3 lysates, rat Brain lysates using Ubiquitin antibody., Immunohistochemistry analysis of paraffin-embedded Human Breast Carcinoma Tissue using Ubiquitin antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval., Immunohistochemistry analysis of paraffin-embedded Human Stomach Carcinoma Tissue using Ubiquitin antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Alternative Name: FLJ25987; MGC8385; ubiquitin B; Ubiquitin; UBCEP1; UBCEP2; RPS27A

Immunogen: Synthetic peptide conjugated to KLH.

Conjugate: Unconjugated

Modification: Unmodified