Ubiquitin Conjugating Enzyme E2 C Monoclonal Antibody-MB12292

Ubiquitin Conjugating Enzyme E2 C Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB12292-50, MB12292-100

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Swiss-Prot: O00762

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-F, IHC-P, ICC/IF, IP,

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20

Background: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 20 kDa; Observed MW: 20 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunocytochemistry analysis of Ubiquitin Conjugating Enzyme E2 C in Hela using Ubiquitin Conjugating Enzyme E2 C antibody, and DAPI., Western blot analysis of UBE2C in PC-12, Raw264.7, 3T3 lysates using UBE2C antibody., Western blot analysis of UBE2C in A549, HL-60, U2OS lysates using UBE2C antibody, Immunohistochemistry analysis of paraffin-embedded Human tonsil using UBE2C antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Alternative Name: UBCH 10; UbcH10; Ube2c

Immunogen: Recombinant protein of human UBE2C

Conjugate: Unconjugated

Modification: Unmodified