Ubiquitin D Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS91402-50, BS91402-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: O15205(Human) P63072(Mouse)
Host: Rabbit
Reactivity: Human, Mouse
Applications: WB, ICC IHC
All Applications: WB:1:1,000-1:2,000
ICC:1:100-1:500
IHC:1:50-1:200
Background: FAT10, also designated Ubiquitin D or Diubiquitin, is a 165 amino acid protein encoded in the major histocompatibility complex (MHC) that consists of two domains which share significant homology with ubiquitin. Each domain contains two cysteines, along with a free C-terminal diglycine motif required for FAT10 conjugate formation. FAT10 is inducible by interferon-g and tumor necrosis factor a (TNF?). The FAT10 protein interacts with MAD2, a component of the spindle checkpoint, and plays a role in antigen presentation, cytokine response, apoptosis and mitosis. It may also regulate cell growth during dendritic cell or B cell activation and development. FAT10 mRNA is expressed mainly in some dendritic cells and lymphoblastoid lines and in other specific cells subsequent to interferon-g induction. The human FAT10 gene, designated UBD, maps to chromosome 6p21.3 and is overexpressed in the tumors of various epithelial cancers.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: Ubiquitin D polyclonal antibody detects endogenous levels of Ubiquitin D protein.
Bioworld Molecular Weight: 18 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Ubiquitin D on Hela cells lysates using anti-Ubiquitin D antibody at 1/1,000 dilution., ICC staining Ubiquitin D in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: UBD, Diubiquitin, FAT10, GABBR1, UBD 3, Ubd, UBD, Ubiquitin D, Ubiquitin like protein FAT10, Ubiquitin-like protein FAT10,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified