Ubiquitin K48 Monoclonal Antibody-MB11135

Ubiquitin K48 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB11135-50, MB11135-100

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Swiss-Prot: P0CG47

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-F, IHC-P, ICC/IF

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200

Background: Plays an important role in the ubiquitin-proteasome pathway. Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the activation component E1; the activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, then from E2 to ubiquitin ligase E3 for final delivery to the epsilon-NH2 of the target protein lysine residue.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of K48 Linkage Specific Ubiquitin in C6, C2C12 lysates using Ubiquitin K48 antibody., Western blot analysis of K48 Linkage Specific Ubiquitin in 293 lysates using K48 Linkage Specific Ubiquitin antibody., Immunohistochemistry analysis of paraffin-embedded Human colon cancer using K48 Linkage Specific Ubiquitin antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Alternative Name: FLJ25987; MGC8385; ubiquitin B; Ubiquitin; UBCEP1; UBCEP2; RPS27A

Immunogen: A synthetic peptide of human K-48 Linkage Specific Ubiquitin

Conjugate: Unconjugated

Modification: Unmodified