Ubiquitin K63 Monoclonal Antibody-MB12298

Ubiquitin K63 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB12298-50, MB12298-100

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Swiss-Prot: P0CG47

Host: Rabbit

Reactivity: Human

Applications: WB, IHC-F, IHC-P, ICC/IF

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200

Background: Plays an important role in the ubiquitin-proteasome pathway. Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the activation component E1; the activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, then from E2 to ubiquitin ligase E3 for final delivery to the epsilon-NH2 of the target protein lysine residue.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 26 kDa; Observed MW: 8 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Ubiquitin in Hela, A549 lysates using Ubiquitin K63 antibody., Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Ubiquitin antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval., Immunocytochemistry analysis of Ubiquitin in hela using Ubiquitin antibody, and DAPI

Alternative Name: FLJ25987; MGC8385; ubiquitin B; Ubiquitin; UBCEP1; UBCEP2; RPS27A

Immunogen: A synthetic peptide of human Ubiquitin (linkage-specific K63)

Conjugate: Unconjugated

Modification: Unmodified