Product Description

Ubiquitin Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91400-50, BS91400-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P0CG48

Host: Rabbit

Reactivity: Human, Mouse, rat

Applications: WB, ICC IHC

All Applications: WB:1:500-1:1,000
ICC:1:200
IHC:1:200

Background: Ubiquitin is a conserved polypeptide unit that plays an important role in the ubiquitin-proteasome pathway. Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B.

Purification and Purity: Peptide affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Ubiquitin polyclonal antibody detects endogenous levels of Ubiquitin protein.

Bioworld Molecular Weight: 10 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Ubiquitin on different cell lysates using anti-Ubiquitin antibody at 1/1000 dilution.
Positive control: Lane 1: 293T Lane 2: HepG2 Lane 3: Hela Lane 4: MCF-2, ICC staining Ubiquitin in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: UBC, Epididymis secretory protein Li 50, FLJ25987, HEL S 50, MGC8385, Polyubiquitin B, RPS 27A, RPS27A, UBA 52, UBA 80, UBA52, UBA80, UBB, UBB, UBC, UBCEP 1, UBCEP 2, UBCEP1, UBCEP2, Ubiquitin, Ubiquitin B,

Immunogen: peptide

Conjugate: Unconjugated

Modification: Unmodified