Product Description

Ubiquitin Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BZ16631-50, BZ16631-100

Product: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Swiss-Prot: P0CG47

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-P, ICC/IF, FC

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 FC: 1/50-1/100

Background: Plays an important role in the ubiquitin-proteasome pathway. Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the activation component E1; the activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, then from E2 to ubiquitin ligase E3 for final delivery to the epsilon-NH2 of the target protein lysine residue.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 26 kDa; Observed MW: 8 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Ubiquitin in 293T lysates using Ubiquitin antibody., Immunohistochemistry analysis of paraffin-embedded Human colon using Ubiquitin antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval., Immunofluorescence analysis of Ubiquitin in Raji using Ubiquitin antibody.

Alternative Name: FLJ25987; MGC8385; ubiquitin B; Ubiquitin; UBCEP1; UBCEP2; RPS27A

Immunogen: A synthesized peptide derived from human Ubiquitin

Conjugate: Unconjugated

Modification: Unmodified