Ubiquitin Rabbit Monoclonal Antibody-BS9922M

Ubiquitin Rabbit Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS9922M-50, BS9922M-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P0CG47/P0CG48/P62979/P62987

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC, FC

All Applications: WB: 1:1000-1:2000
ICC: 1:50-1:200
IHC: 1:200-1:500
FC: 1:50-1:100

Background: Ubiquitin (Ub) is among the most phylogenetically conserved proteins known. The primary function of ubiquitin is to clear abnormal, foreign and improperly folded proteins by targeting them for degradation by the 26S proteosome. This small, 76 amino acid protein can be covalently attached to cellular proteins via an isopeptide linkage between the carboxy terminal group of ubiquitin and lysine amino groups on the acceptor protein. For proteolysis to occur, ubiquitin oligomers must be assembled. Ubiquitin chains on proteolytic substrates are commonly found to have an isopeptide bridge between Lysine 48 of one ubiquitin molecule and the carboxy-terminus of a neighboring ubiquitin molecule.

Purification and Purity: Protein A affinity purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects ubiquitin, polyubiquitin and ubiquitinated proteins.

Bioworld Molecular Weight: ~ *8 kDa or Uncertain

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Ubiquitin Rabbit mAb at 1:1000 dilution
Lane 1: The heart tissue lysate of Mouse, Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Ubiquitin antibody. Counter stained with hematoxylin., ICC staining Ubiquitin in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.

Alternative Name: Polyubiquitin-B; Ubiquitin; UBB; Polyubiquitin-C; UBC; RPS27A; UBA80; UBCEP1; CEP52; UBA52; UBCEP2

Immunogen: Recombinant antibody.

Conjugate: Unconjugated

Modification: Unmodified