UCHL5 Polyclonal Antibody-BS71027

UCHL5 Polyclonal Antibody

Sizes: 50 µl, 100 µl

Catalogue Numbers: BS71027-50, BS71027-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9Y5K5

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:100

Background: Uch37 is one of the deubiquitinating enzymes of the human 26S proteasome, belongs to the Ub C-terminal hydrolases. Uch37 was found to bind both Adrm1 and S5a subunits of the 26S proteasome. Uch37 likely contributes to the Ub chain trimming activity of the 26S proteasome that shortens a polyUb chain from the distal end of the chain.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 38KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of various lysates, using UCHL5 antibody at 1:433 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s., Immunofluorescence analysis of NIH/3T3 cells using UCHL5 Rabbit pAb at dilution of 1:50. Blue: DAPI for nuclear staining.

Alternative Name: UCHL5; CGI-70; INO80R; UCH-L5; UCH37

Immunogen: Recombinant fusion protein of human UCHL5(NP_001186190.1).

Conjugate: Unconjugated

Modification: Unmodified