UDG (H221) Polyclonal Antibody-BS2465

UDG (H221) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS2465-50, BS2465-100

Sizes: 50µl, 100µl

Swiss-Prot: P13051

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: The human UNG-gene encodes nuclear (UNG2) and mitochondrial (UNG1) forms of uracil-DNA glycosylase using differentially regulated promoters, PA and PB, and alternative splicing to produce two proteins with unique N-terminal sorting sequences. Uracil in DNA may result from deamination of cytosine which can give rise to transition mutations. Uracil-DNA glycosylase is the DNA repair enzyme responsible for the removal of uracil from DNA. This is probably a biologically important function in the prevention of mutagenesis resulting from cytosine deamination. UNG2 removes misincorporated dUMP residues.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: UDG (H221) polyclonal antibody detects endogenous levels of UDG protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of UDG (H221) pAb at 1:500 dilution
Lane 1: Hela whole cell lysate (40ug)
Lane 2: MCF-7 whole cell lysate (40ug)
Lane 3: SP2/0 whole cell lysate (40ug)
Lane 4: H9C2 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of UDG (H221) pAb in paraffin-embedded human skeletal muscle tissue.

Bioworld Molecular Weight: ~ 35 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Uracil-DNA glycosylase; UDG; UNG; DGU; UNG1; UNG15

Immunogen: Synthetic peptide, corresponding to amino acids 200-240 of Human UDG.

Conjugate: Unconjugated

Modification: Unmodified