UGCG Polyclonal Antibody
Catalogue Numbers: BS60390-50, BS60390-100
Sizes: 50µl, 100µl
Alternative Name: Ceramide glucosyltransferase; GLCT-1; GLCT1; Glucosylceramide synthase; GCS; UDP-glucose ceramide glucosyltransferase; UDP-glucose:N-acylsphingosine D-glucosyltransferase
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q16739
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB
All Applications: WB: 1:500~1:1000
Background: Glucosylceramide synthase (GCS), also designated ceramide glucosyltransferase, belongs to the glycosyltransferase 2 family. It is a widely expressed integral membrane protein encoded by UGCG. The enzyme can be found in the plasma membrane of all eukaryotic cells, and a significant concentration of glucosylceramide synthase activity has been reported in the Golgi complex. Glucosylceramide synthase catalyzes the first glycosylation step in glycosphingolipid biosynthesis and functions as a glucosyltransferase and flippase in the transfer of glucose to ceramide. Glucosylceramide synthase operates in cell recognition, cell proliferation and differentiation, immune recognition and signal transduction. The regulation of ceramide levels through glucosylceramide synthase has been associated with the induction of apoptosis and notable research implicates this relationship with drug-induced apoptosis in a variety of cell types.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: UGCG polyclonal antibody detects endogenous levels of UGCG protein.
Bioworld Molecular Weight: ~ 48 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of UGCG polyclonal antibody at 1:500 dilution
Lane 1: Hela cell lysate
Lane 2: Raw264.7 cell lysate
Lane 3: PC12 cell lysate
Immunogen: A synthetic peptide corresponding to residues in Human UGCG.
Conjugate: Unconjugated
Modification: Unmodified