UGT1A10 Polyclonal Antibody Sizes: 50µl, 100µl Catalogue Numbers: BS76841-50, BS76841-100 Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Swiss-Prot: Q9HAW8 Host: Rabbit Reactivity: Human Applications: WB All Applications: WB,1:500 - 1:2000 Background: This gene encodes a UDP-glucuronosyltransferase, an enzyme of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. This gene is part of a complex locus that encodes several UDP-glucuronosyltransferases. The locus includes thirteen unique alternate first exons followed by four common exons. Four of the alternate first exons are considered pseudogenes. Each of the remaining nine 5' exons may be spliced to the four common exons, resulting in nine proteins with different N-termini and identical C-termini. Each first exon encodes the substrate binding site, and is regulated by its own promoter. The enzyme encoded by this gene has glucuronidase activity on mycophenolic acid, coumarins, and quinolines. Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity: Unmodification Extra Notes: Western blot analysis of extracts of various cell lines, using UGT1A10 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s. Note: For research use only, not for use in diagnostic procedure. Bioworld Molecular Weight: 60kDa Alternative Name: UGT1A10; UDPGT; UGT-1J; UGT1-10; UGT1.10; UGT1J Immunogen: A synthetic peptide of human UGT1A10(NP_061948.1). Conjugate: Unconjugated Modification: Unmodified