Product Description

ULK3 Monoclonal Antibody

Product: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Catalogue Numbers: MB67135-50, MB67135-100

Sizes: 50µl, 100µl

Swiss-Prot: Q6PHR2

Host: Mouse

Reactivity: Human, Mouse, Rat

Applications: WB

Background: Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy.

Product: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification and Purity: This antibody is purified through a protein G column.

Specificity: Recognizes endogenous levels of ULK3 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of ULK3 expression in Hela (A), LNCaP (B), mouse testis (C), rat testis (D) whole cell lysates., Immunohistochemical analysis of ULK3 staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Bioworld Molecular Weight: ~ 55 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Serine/threonine-protein kinase ULK3; Unc-51-like kinase 3

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human ULK3. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified