Product Description

UMP-CMP Kinase (E36) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS1975-50, BS1975-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P30085

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IP,

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
IP: 1:10~1:100

Background: Nucleoside monophosphate kinases are required for pharmacological activation of therapeutic nucleosides and nucleotide analogs. CMPK (Cytidine monophosphate kinase), also known as UMP-CMP kinase and Deoxycytidylate kinase, is a 196 amino acid protein that catalyzes the phosphoryl transfer from ATP to UMP, CMP and dCMP. This enzymatic reaction leads to the formation of ADP and the corresponding nucleoside diphosphate, which are required for cellular nucleic acid synthesis. Primarily localized to the cytoplasm, CMPK also plays an important role in the activation of pyrimidine analogs, which is clinically useful anti-cancer and anti-viral drugs. CMP is the best substrate for CMPK, followed by UMP and dCMP.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: UMP-CMP Kinase (E36) polyclonal antibody detects endogenous levels of UMP-CMP Kinase protein.

Bioworld Molecular Weight: ~ 26 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of UMP-CMP Kinase (E36) pAb at 1:1000 dilution
Lane 1: Hela whole cell lysate (40ug)
Lane 2: H9C2 whole cell lysate (40ug)
Lane 3: MEF whole cell lysate (40ug)
Lane 4: A549 whole cell lysate (40ug)
Lane 5: HEK293T whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of UMP-CMP Kinase (E36) pAb in paraffin-embedded human breast carcinoma tissue at 1:100., Immunoprecipitation of HEK293T cell lysate using UMP-CMP Kinase (E36) pAb (Sepharose Bead Conjugate) #BD0048 (Lane 2 and Lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate) #BD0048 (Lane 4 and Lane 5). Lane 1 is 30% input. The western blot was probed using UMP-CMP Kinase (E36) #BS1975. “↑”（supernatant); “↓(deposition）

Alternative Name: UMP-CMP kinase; Deoxycytidylate kinase; dCMP kinase; Nucleoside-diphosphate kinase; Uridine monophosphate/cytidine monophosphate kinase; UMP/CMP kinase; UMP/CMPK; CMPK1; CMK; CMPK; UCK; UMK; UMPK

Immunogen: Synthetic peptide, corresponding to the N-terminal of Human UMP-CMP Kinase.

Conjugate: Unconjugated

Modification: Unmodified