URB1 Polyclonal Antibody
Catalogue Numbers: BS61228-50, BS61228-100
Sizes: 50µl, 100µl
Alternative Name: Nucleolar pre-ribosomal-associated protein 1; Nucleolar protein 254 kDa; URB1 ribosome biogenesis 1 homolog; URB1; C21orf108; KIAA0539; NOP254; NPA1
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: O60287
Host: Rabbit
Reactivity: Human
Applications: WB
All Applications: WB: 1:500~1:1000
Background: Real-time GlaI-PCR assay is developed to determine DNA methylation status of the regulation regions of HDAC4, URB1 and RARB genes in DNA preparations from human leukocytes. Real-time GlaI-PCR assay is DNA hydrolysis with methyl-directed site-specific DNA endonuclese GlaI followed by real-time PCR from the primers located upstream and downstream of the studied DNA region.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: URB1 pAb detects endogenous levels of URB1 protein.
Bioworld Molecular Weight: ~ 254 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of URB1 pAb at 1:500 dilution
Lane 1: Panc1 whole cell lysate(20ug)
Lane 2: SGC7901 whole cell lysate(20ug)
Lane 3: MCF-7 whole cell lysate(40ug)
Immunogen: Synthetic peptide, corresponding Human URB1.
Conjugate: Unconjugated
Modification: Unmodified