USP14 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS91412-50, BS91412-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P54578(Human)
Host: Rabbit
Reactivity: Human
Applications: WB, FC
All Applications: WB:1:500-1:1,000
FC:1:50-1:100
Background: Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome. Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. Indispensable for synaptic development and function at neuromuscular junctions. Plays a role in the innate immune defense against viruses by stabilizing the viral DNA sensor CGAS and thus inhibiting its autophagic degradation.
Purification and Purity: Protein affinity purified.
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Specificity: USP14 polyclonal antibody detects endogenous levels of USP14 protein.
Bioworld Molecular Weight: 56 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of USP14 on Daudi cell lysate using anti-USP14 antibody at 1/500 dilution., Flow cytometric analysis of SiHa cells with USP14 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody;black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Alternative Name: USP14, TGT, Deubiquitinating enzyme 14, TGT, tRNA guanine transglycosylase 60 kD subunit, tRNA guanine transglycosylase, Ubiquitin carboxyl terminal hydrolase 14, Ubiquitin carboxyl-terminal hydrolase 14, Ubiquitin specific peptidase 14, Ubiquitin specific processing protease 14, Ubiquitin specific protease 14, Ubiquitin thiolesterase 14, Ubiquitin-specific-processing protease 14, UBP14, USP 14, USP14,
Immunogen: Recombinant protein within human USP14 aa 200-450.
Conjugate: Unconjugated
Modification: Unmodified