USP22 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS91415-50, BS91415-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q9UPT9(Human)
Host: Rabbit
Reactivity: Human
Applications: WB, ICC/IF FC IP,
All Applications: WB:1:1,000-1:5,000
ICC:1:50-1:200
FC:1:50-1:100
IP:1:10-1:50
Background: The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP22 (ubiquitin specific peptidase 22), also known as USP3L, is a 525 amino acid protein that contains one UBP-type zinc finger and functions to catalyze the conversion of a ubiquitin C-terminal thioester to free ubiquitin and thiol, a reaction that may influence several cellular processes. Via its catalytic activity, USP22 is thought to play an important role in cell cycle progression and may also serve as a cancer stem cell marker. The gene encoding USP22 maps to human chromosome 17, which comprises over 2.5% of the human genome and encodes over 1,200 genes.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: USP22 polyclonal antibody detects endogenous levels of USP22 protein.
Bioworld Molecular Weight: 60 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of USP22 on Hela (1) and HepG2 (2) cell lysate using anti-USP22 antibody at 1/500 dilution., ICC staining USP22 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: USP22, KIAA1063, USP3L, Deubiquitinating enzyme 22, KIAA1063, Ubiquitin carboxyl terminal hydrolase 22, Ubiquitin carboxyl-terminal hydrolase 22, Ubiquitin specific peptidase 22, Ubiquitin specific peptidase 3 like, Ubiquitin specific processing protease 22, Ubiquitin specific protease 22, Ubiquitin thioesterase 22, Ubiquitin thiolesterase 22, Ubiquitin-specific-processing protease 22, UBP22, USP 22, Usp22, USP3L,
Immunogen: Recombinant protein
Conjugate: Unconjugated
Modification: Unmodified