Product Description

USP30 Polyclonal Antibody

Catalogue Numbers: BS65002-50, BS65002-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Swiss-Prot: Q70CQ3

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP30 (ubiquitin specific peptidase 30) is a 517 amino acid protein that localizes to the mitochondrion and belongs to the ubiquitin-specific protease family.
Expressed in pancreas, kidney, liver and skeletal muscle, USP30 functions to catalyze the conversion of a ubiquitin C-terminal thioester to a free ubiquitin and a thiol. The gene encoding USP30 maps to human chromosome 12q24.11, which encodes over 1,100 genes and comprises approximately 4.5% of the human genome.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: USP30 polyclonal antibody detects endogenous levels of USP30 protein.

Extra Notes: Western blot (WB) analysis of USP30 polyclonal antibody at 1:500 dilution
Lane1:Aml-12 whole cell lysate(40ug)
Lane2:H9C2 whole cell lysate(40ug)
Lane3:HEK293T whole cell lysate(20ug)
Lane4:The muscle tissue lysate of mouse(40ug)
Lane5:Panc1 whole cell lysate(20ug)
Lane6:HepG2 whole cell lysate(20ug)

Bioworld Molecular Weight: ~ 60kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Ubiquitin carboxyl-terminal hydrolase 30; Deubiquitinating enzyme 30; Ubiquitin thioesterase 30; Ubiquitin-specific-processing protease 30; Ub-specific protease 30; USP30

Immunogen: Synthetic peptide, corresponding to Human USP30.

Conjugate: Unconjugated

Modification: Unmodified