Product Description

USP7/HAUSP Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS75377-50, BS75377-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q93009

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF/ICC

All Applications: WB,1:500 - 1:2000 | IHC,1:50 - 1:100 | IF/ICC,1:50 - 1:100

Background: The protein encoded by this gene belongs to the peptidase C19 family, which includes ubiquitinyl hydrolases. This protein deubiquitinates target proteins such as p53 (a tumor suppressor protein) and WASH (essential for endosomal protein recycling), and regulates their activities by counteracting the opposing ubiquitin ligase activity of proteins such as HDM2 and TRIM27, involved in the respective process. Mutations in this gene have been implicated in a neurodevelopmental disorder.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 138kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using USP7/HAUSP antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s., Immunohistochemistry of paraffin-embedded rat testis using USP7/HAUSP antibody at dilution of 1:100. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded mouse testis using USP7/HAUSP antibody at dilution of 1:100. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded mouse brain using USP7/HAUSP antibody at dilution of 1:100. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Alternative Name: USP7; HAUSP; TEF1

Immunogen: Recombinant fusion protein of human USP7/HAUSP(NP_003461.2).

Conjugate: Unconjugated

Modification: Unmodified