USP9x Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS91419-50, BS91419-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q93008(Human) P70398(Mouse)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC, IF, IHC, FC
All Applications: WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
Background: Deubiquitinase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. May therefore play an important regulatory role at the level of protein turnover by preventing degradation of proteins through the removal of conjugated ubiquitin. Specifically hydrolyzes 'Lys-48'-, 'Lys-29'- and 'Lys-33'-linked polyubiquitins chains. Essential component of TGF-beta/BMP signaling cascade. Specifically deubiquitinates monoubiquitinated SMAD4, opposing the activity of E3 ubiquitin-protein ligase TRIM33. Deubiquitinates alkylation repair enzyme ALKBH3. OTUD4 recruits USP7 and USP9X to stabilize ALKBH3, thereby promoting the repair of alkylated DNA lesions. Regulates chromosome alignment and segregation in mitosis by regulating the localization of BIRC5/survivin to mitotic centromeres. Involved in axonal growth and neuronal cell migration.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Specificity: USP9x polyclonal antibody detects endogenous levels of USP9x protein.
Bioworld Molecular Weight: 292 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of USP9X on different lysates using anti-USP9X antibody at 1/1,000 dilution.
Positive control:
Lane 1: SiHa
Lane 2: A549
Lane 3: 293
Lane 4: Mouse colon, ICC staining USP9X in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: hFAM, USP9X, DFFRX, FAM, USP9, Deubiquitinating enzyme FAF X, Deubiquitinating enzyme FAF-X, DFFRX, Drosophila fat facets related X linked, FAF, Fafl, Fam, Fat facets homolog, Fat facets in mammals, Fat facets protein related X linked, Fat facets protein related, X-linked, Fat facets protein-related, hFAM, MRX99, Probable ubiquitin carboxyl terminal hydrolase FAF X, Probable ubiquitin carboxyl-terminal hydrolase FAF-X, Ubiquitin carboxyl-terminal hydrolase FAM, Ubiquitin specific peptidase 9 X linked, Ubiquitin specific peptidase 9, X-linked, Ubiquitin specific processing protease FAF X, Ubiquitin specific protease 9 X chromosome, Ubiquitin thioesterase FAF X, Ubiquitin thiolesterase FAF X, Ubiquitin thiolesterase FAF-X, Ubiquitin-specific protease 9, Ubiquitin-specific-processing protease FAF-X, USP9 (gene name), Usp9x, USP9X, Uubiquitin specific protease 9, X chromosome (fat facets like Drosophila), X chromosome, X-linked,
Immunogen: Recombinant protein within human USP9X aa 1-200.
Conjugate: Unconjugated
Modification: Unmodified