Product Description

VAMP8 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91422-50, BS91422-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9BV40(Human) O70404(Mouse) Q9WUF4(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC, IP, , FC

All Applications: WB:1:1,000-1:2,000
ICC:1:50-1:100
IHC:1:50-1:200
FC:1:50-1:100

Background: Syntaxins were originally thought to be docking proteins, but have more recently been categorized as anchoring proteins that anchor themselves to the cytoplasmic surfaces of cellular membranes. Syntaxins bind to various proteins involved in exocytosis, including VAMPs (vesicle-associated membrane proteins), NSF (N-ethylmaleimide-sensitive factor), SNAPs (soluble NSF attachment proteins) and Synaptotagmin. Endobrevin, also designated VAMP-8 or ED, is a 100 amino acid single-pass type IV membrane protein that belongs to the synaptobrevin family. Similar in sequence to the synaptobrevins, endobrevin is abundantly expressed in kidney, moderately expressed in heart and spleen, and slightly expressed in brain, thymus and liver. Endobrevin interacts specifically with the SNAPs, most likely through an endobrevin-containing SNARE complex.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: VAMP8 polyclonal antibody detects endogenous levels of VAMP8 protein.

Bioworld Molecular Weight: 11 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of VAMP8 on different lysates using anti-VAMP8 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: Mouse kidney, ICC staining VAMP8 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: VAMP-8, EDB, VAMP8, EDB, Endobrevin, VAMP 8, VAMP-8, VAMP8, VAMP8, Vesicle associated membrane protein 8, Vesicle-associated membrane protein 8,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified