Product Description

VAV2 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91424-50, BS91424-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P52735(Human) Q60992(Mouse) EntrezGene:296603(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC, IHC, IP,

All Applications: WB:1:1,000-1:5,000
ICC:1:50-1:200
IHC:1:50-1:200

Background: The Vav gene was originally identified on the basis of its oncogenic activation during the course of gene transfer assays. The major translational product of the Vav proto-oncogene has been identified as a protein containing an array of structural motifs. This protein, known as Vav, Vav1 or p95Vav, contains an N-terminal helix-loop-helix domain and a leucine zipper motif similar to that of Myc family proteins that, if deleted, causes oncogenic activation. In addition, Vav contains an SH2 domain, which could indicate its role as a substrate for tyrosine kinases. Expression of Vav is limited exclusively to cells of hematopoietic origin, including those of the erythroid, lymphoid and myeloid lineages. These results suggest that Vav may represent a new type of signal transduction molecule involved in the transduction of tyrosine phosphorylation signaling into transcriptional events. Vav2 is a member of the Vav family of oncoproteins and acts as a guanosine nucleotide exchange factor (GEF) for RhoG and RhoA-like GTPases in a phosphotyrosine-dependent manner.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: VAV2 polyclonal antibody detects endogenous levels of VAV2 protein.

Bioworld Molecular Weight: 100 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of VAV2 on SW480 cells lysates using anti-VAV2 antibody at 1/1,000 dilution., ICC staining VAV2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: VAV-2, VAV2, Guanine nucleotide exchange factor VAV2, Oncogene VAV2, Protein vav 2, VAV 2, Vav 2 oncogene, VAV-2, VAV2, VAV2 oncogene, VAV2,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified