VCAM1 Polyclonal Antibody Sizes: 50µl, 100µl Catalogue Numbers: BS66143-50, BS66143-100 Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Swiss-Prot: P19320 Host: Rabbit Reactivity: Human, Mouse, Rat Applications: WB, ELISA IHC IF All Applications: WB: 1:500-1:2000 ELISA: 1:5000-1:10000 IHC: 1:100-1:500 IF: 1:100-1:500 Background: The intracellular adhesion molecule-1 (ICAM-1), also referred to as CD54, is an integral membrane protein of the immunoglobulin superfamily and recognizes the B2α1 and B2αM integrins. PECAM-1 (platelet/endothelial cell adhesion molecule-1), also referred to as CD31, is a glycoprotein expressed on the cell surfaces of monocytes, neutrophils, platelets and a subpopulation of T cells. VCAM-1 (vascular cell adhesion molecule-1) was first identified as an adhesion molecule induced on human endothelial cells by inflammatory cytokines such as IL-1, tumor necrosis factor (TNF) and lipopolysaccharide (LPS). The KALIG gene encodes a nerve cell adhesion molecule (NCAM) -like protein and is deleted in 66% of patients with Kallmann's syndrome, anosmia with secondary hypogonadism . Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity: VCAM1 polyclonal antibody detects endogenous levels of VCAM1 protein. Bioworld Molecular Weight: ~ 81, 110 kDa Note: For research use only, not for use in diagnostic procedure. Extra Notes: Primary: Anti-VCAM-1 at 1/1000 dilution, Primary: Anti-VCAM-1 at 1/300 dilution, Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-VCAM-1 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C. Alternative Name: Vascular cell adhesion protein 1; V-CAM 1; VCAM-1; INCAM-100; CD106; L1CAM Immunogen: KLH conjugated synthetic peptide derived from mouse VCAM-1: 640-739/739 Conjugate: Unconjugated Modification: Unmodified